Beyene et al.: Saliva has higher yield in detecting SARS-CoV2, and COVID-19 patients show higher viral load and prolonged period of viral shedding in saliva.
Beyene et al.: Saliva has higher yield in detecting SARS-CoV2, and COVID-19 patients show higher viral load and prolonged period of viral shedding in saliva.
I’ve seen concerning videos in the past about the swabs. Nanotechnology and/or grapheme oxide in the swabs. All seemed a little too crazy to consider. We’ve learned anything is possible now. I’ve avoided testing because of it. I wondered why swabs weren’t oral, it’s less invasive, certainly easier for kiddos. This just brings more questions.
This crap has been unleashed on the world. The vaccine kills, some quickly others will be a slow process, heart disease, cancer’s and painful autoimmune conditions that may leave victims damaged for years.
I’ve tested ONCE in my entire life. I wouldn’t even test for my job! Here in Commifornia, you are required to test to be admitted into a hospital. It’s ridiculous! I had a cancerous tumor and I’m not messing with THAT! But, it was barely put in my nose. I was really surprised. The whole thing is a big SCAM!
Is anyone still testing? Didn’t the latest CDC guidelines make testing useless, which it has been from the beginning……it was a fear tactic and remains that. Worthless. If you don’t feel good stay at home until you feel better. Same thing the world has been doing for the flu virus forever.
My impression is that the variants have tended, in general, to manifest themselves increasingly in the mouth and throat more than the nose. For example, sore throat has become a more common symptom since the original variant. I have wondered whether this might be an evolutionary adaption by the virus that has been selected because it has made it easier to escape early detection by nasal swabs.
Oh dear. You wrote: detecting SARS-CoV2 in COVID-19 patients. ... NO.
Methods:
RNA extraction
For both types of samples, NPS and saliva, viral nucleic acid (NA) was extracted from a volume 200 μL using the NA extraction and purification reagent, DAAN Gene Co., Ltd, as recommended by the manufacturer (Da An Gene Co., Ltd, of Sun Yat-Sen University, China). Briefly, 50 μL proteinase K, and 200 μL lysis buffer were mixed with 200 μL NPS and/or saliva samples. Then, the lysed samples were heat inactivated on dry heat block at 72 °C for 10 min, followed by the addition of inhibitor remover and subsequent washing. Finally, the NA was eluted in 50 μL molecular grade water preheated at 72 °C.
Not a "virus" in sight. All indirect sleight of hand and assumption.
A polynucleotide sequence of manifest physiological breakdown more likely.
I’ve seen concerning videos in the past about the swabs. Nanotechnology and/or grapheme oxide in the swabs. All seemed a little too crazy to consider. We’ve learned anything is possible now. I’ve avoided testing because of it. I wondered why swabs weren’t oral, it’s less invasive, certainly easier for kiddos. This just brings more questions.
This crap has been unleashed on the world. The vaccine kills, some quickly others will be a slow process, heart disease, cancer’s and painful autoimmune conditions that may leave victims damaged for years.
Lord help us.
Collecting saliva is something which can be done where all one needs to do is spit in a vessel of some sort.
As long as the pcr is <22ct and is conducted by qualified lab technicians in a truly sterile environment.
Given past history I doubt they can get the above conditions in place and will go for the false positive scamdemic route. John 8:44
I’m done testing. I never did it in the first place. I’m not going to start. This is just ridiculous.
So, if they use high cycle PCR tests, will the end result yield more positives? 🤔
I’ve tested ONCE in my entire life. I wouldn’t even test for my job! Here in Commifornia, you are required to test to be admitted into a hospital. It’s ridiculous! I had a cancerous tumor and I’m not messing with THAT! But, it was barely put in my nose. I was really surprised. The whole thing is a big SCAM!
Is anyone still testing? Didn’t the latest CDC guidelines make testing useless, which it has been from the beginning……it was a fear tactic and remains that. Worthless. If you don’t feel good stay at home until you feel better. Same thing the world has been doing for the flu virus forever.
My impression is that the variants have tended, in general, to manifest themselves increasingly in the mouth and throat more than the nose. For example, sore throat has become a more common symptom since the original variant. I have wondered whether this might be an evolutionary adaption by the virus that has been selected because it has made it easier to escape early detection by nasal swabs.
Oh dear. You wrote: detecting SARS-CoV2 in COVID-19 patients. ... NO.
Methods:
RNA extraction
For both types of samples, NPS and saliva, viral nucleic acid (NA) was extracted from a volume 200 μL using the NA extraction and purification reagent, DAAN Gene Co., Ltd, as recommended by the manufacturer (Da An Gene Co., Ltd, of Sun Yat-Sen University, China). Briefly, 50 μL proteinase K, and 200 μL lysis buffer were mixed with 200 μL NPS and/or saliva samples. Then, the lysed samples were heat inactivated on dry heat block at 72 °C for 10 min, followed by the addition of inhibitor remover and subsequent washing. Finally, the NA was eluted in 50 μL molecular grade water preheated at 72 °C.
Not a "virus" in sight. All indirect sleight of hand and assumption.
A polynucleotide sequence of manifest physiological breakdown more likely.
Take your choice on causal attribution.
I put together a post on this if anyone is interested in the anatomy and complications of nasal swabs:
https://medquotes.substack.com/p/to-the-nose-and-beyond