tRNA (transfer RNA) is critical to the translation to proteins (e.g. spike protein) from mRNA & there are 3 STOP codons UGA, UAA, & UAG that function to stop the translation (protein synthesis);
tRNA (transfer RNA) is critical to the translation to proteins (e.g. spike protein) from mRNA & there are 3 STOP codons UGA, UAA, & UAG that function to stop the translation (protein synthesis);
Did the swapping out of URACIL (the initial uridine) for a pseudouridine (Ψ) (methylated-pseudouridine) cause the translation (building) of spike protein to be non-stop (24/7) in all cells?
The complexity of the human cell, which carries the blueprint for life, dictates, that we do not interfere with normal cellular function by reprogramming normal cell processes! Stop the injections now. Save our children now.! EAU mandate has allowed humans to become lab rats for the profitability of BigPharma!
I wonder if spike is made to continuously produce BUT that the mRNA code can be switched out meaning at any given time a different protein can be produced if given the new code somehow.
Dr. Alexander, it seems to me that you are asking the wrong people, most of us are not scientists. You seem to be on the right track & you certainly have colleagues that you can work with to come up with the answer. This is horrifying on so many levels. If shedding is real then this means we that have been so careful not to take the shot cannot ever get together with anyone who has. And/or it doesn’t matter because we will be contaminated anyway just by being exposed to 70% of the population. It also means that the shot is really a death sentence, it’s just a matter of time.
This type of analysis is exactly the thing we need in order to have informed consent. You bring real science to a cabal-corrupted medical regime made up of … let’s call them what they are … deceivers and parasites. Dr. Alexander, you need to head one of these agencies in the next Trump administration. Blessings
I think a misread stop codon and continued protein production from an mRNA molecule are two different questions.
If I remember undergraduate molecular biology correctly, if the stop codon is misread and an amino acid added instead, this will cause read-through and continuation of the polypeptide synthesized during translation of the mRNA. This will proceed until the next stop codon is reached, and is a natural phenomenon under some conditions. So the protein will have additional amino acids that are not intended to be there. I haven't gone back to the spike protein sequence to see which amino acids, or how many, these might be.
During protein synthesis, an mRNA is looped by interactions between proteins that bind the 5'cap at the start and those that bind the 3'UTR at the end. These bring in the ribosome to begin protein synthesis. Other proteins facilitate release of the ribosome once the polypeptide synthesis ends with a stop codon. Whether the ribosome re-engages with the mRNA for another round of protein synthesis depends on a variety of factors. Having a stabilised mRNA that is not readily degraded will likely increase this possibility.
Interesting that the altered uridine is the way they cancelled the "stop". However, they've stated openly that their intention was for the body to produce spike protein, so I don't think we can claim a surreptitious intent (not on those grounds anyway).
I'm interested in your comment that manufacturing spike protein could go on "forever". I'm only aware of studies finding spike persevering in the body for 21 days. Has anyone found it longer than that?
Of course the vaxx -- wheter the spike protein or other components-- is quite capable of killing or creating permanent disability whether it's present longer, or not.
Doc, just a thought 🤔...is there any way to mitigate prior to translation at all? Like cutting it off or weakening it at the path before it has a chance to translate at all? Is that possible?
Also, if it’s a pseudo amino acid being introduced to replace Uracil, could you introduce another “safe pseudo something” to maybe stop it in its tracks? Fight pseudo with pseudo kind of thing?
The paper you linked is about pseudouridine substitution and the Spike mRNA is made with N-methyl-pseudouridine, which unlike pseudouridine has less wobble when base-pairing with a tRNA, and that's why N-methyl-pseudouridine is used instead of pseudouridine. Now that does exclude the possibility of reading through the stop codon (there are actually two in a row), it has not been tested as far as I know (it should have been tested!!!). For the Pfizer mRNA, it would add 61 aa until the next stop codon, each time the probability of read-through would decrease, but it is conceivable a fraction of the translated products have extra tails of amino-acids that should not be there. However, this will not result in more numerous Spike proteins, just extra-length on a fraction of them, whose biological impact is completely unknown. From a molecular biologist.
I don't have academic training but I have an inquiring mind. If our ribosomes evolved to make proteins on request from mRNA that has these specific stop codons, presumably to signal the ribosome that the docket is now fulfilled, is it reasonable to ask whether mRNA without these specific stop codons but with substitute [substandard] codons, can damage the ribosome?
P.S. Personally…I have worked with the NORTH AMERICAN LAW CENTER group for 15 years and can tell you they are America’s best brilliant and strategic minds!
Please help spread Lex’s pieces…important information ALL Americans need!
Reach out to Lex at his email found at the end of all his pieces to find out how to receive his information!
I wonder if there’s a risk of continued protein production in commercially sterilized fibrin sealants used in surgeries, or is it safe to assume that the “ground to a powder” production process would eliminate the spiked protein?
As production of the COVID-19 "vaccination" by Pfizer was actually an OTA contract between Pfizer and the Department of Defense, Pfizer was only required to produce a "demonstration" product. There was no party responsible for overseeing the entire operation (Trump's Operation Warpspeed) to insure safety and efficacy. The FDA and CDC roles were only a fake rubber stamp of approval. The "vaccine" functions well in its role as a medical countermeasure, aka bioweapon. Obviously, the health of we the people was never intended to be a factor of importance in the production and distribution of this poison. In addition to the world's population being a victim of this act of war, science has been discarded as a benefit to mankind.
My understanding is that the stop codon indicates the end of the protein. So stop and release what has been produced. The mRNA strand is then degraded and another produced from DNA if more protein is needed.
Adding the pseudouridine makes the breakdown of this mRNA strand difficult or impossible. Thus this strand is reused for another copy of protein.
If the cells cannot break it down, what happens to it? Presumably shedding could provide a mechanism for removal from the cells and body. Is there somewhere else in nature that this artificial mRNA will be degraded? I’d expect so, only outside of cell components where protein production occurs.
It makes perfect sense when you come to the horrifying conclusion that this was never about our health.
The complexity of the human cell, which carries the blueprint for life, dictates, that we do not interfere with normal cellular function by reprogramming normal cell processes! Stop the injections now. Save our children now.! EAU mandate has allowed humans to become lab rats for the profitability of BigPharma!
Stop asking Govt Pharma Tech Media Medical Industrial Complex questions instead of science questions.
I wonder if spike is made to continuously produce BUT that the mRNA code can be switched out meaning at any given time a different protein can be produced if given the new code somehow.
Dr. Alexander, it seems to me that you are asking the wrong people, most of us are not scientists. You seem to be on the right track & you certainly have colleagues that you can work with to come up with the answer. This is horrifying on so many levels. If shedding is real then this means we that have been so careful not to take the shot cannot ever get together with anyone who has. And/or it doesn’t matter because we will be contaminated anyway just by being exposed to 70% of the population. It also means that the shot is really a death sentence, it’s just a matter of time.
This type of analysis is exactly the thing we need in order to have informed consent. You bring real science to a cabal-corrupted medical regime made up of … let’s call them what they are … deceivers and parasites. Dr. Alexander, you need to head one of these agencies in the next Trump administration. Blessings
I think a misread stop codon and continued protein production from an mRNA molecule are two different questions.
If I remember undergraduate molecular biology correctly, if the stop codon is misread and an amino acid added instead, this will cause read-through and continuation of the polypeptide synthesized during translation of the mRNA. This will proceed until the next stop codon is reached, and is a natural phenomenon under some conditions. So the protein will have additional amino acids that are not intended to be there. I haven't gone back to the spike protein sequence to see which amino acids, or how many, these might be.
During protein synthesis, an mRNA is looped by interactions between proteins that bind the 5'cap at the start and those that bind the 3'UTR at the end. These bring in the ribosome to begin protein synthesis. Other proteins facilitate release of the ribosome once the polypeptide synthesis ends with a stop codon. Whether the ribosome re-engages with the mRNA for another round of protein synthesis depends on a variety of factors. Having a stabilised mRNA that is not readily degraded will likely increase this possibility.
Interesting that the altered uridine is the way they cancelled the "stop". However, they've stated openly that their intention was for the body to produce spike protein, so I don't think we can claim a surreptitious intent (not on those grounds anyway).
I'm interested in your comment that manufacturing spike protein could go on "forever". I'm only aware of studies finding spike persevering in the body for 21 days. Has anyone found it longer than that?
Of course the vaxx -- wheter the spike protein or other components-- is quite capable of killing or creating permanent disability whether it's present longer, or not.
Doc, just a thought 🤔...is there any way to mitigate prior to translation at all? Like cutting it off or weakening it at the path before it has a chance to translate at all? Is that possible?
Also, if it’s a pseudo amino acid being introduced to replace Uracil, could you introduce another “safe pseudo something” to maybe stop it in its tracks? Fight pseudo with pseudo kind of thing?
This is the kind of post from you that I admire and appreciate greatly. Thank you, Dr!
The paper you linked is about pseudouridine substitution and the Spike mRNA is made with N-methyl-pseudouridine, which unlike pseudouridine has less wobble when base-pairing with a tRNA, and that's why N-methyl-pseudouridine is used instead of pseudouridine. Now that does exclude the possibility of reading through the stop codon (there are actually two in a row), it has not been tested as far as I know (it should have been tested!!!). For the Pfizer mRNA, it would add 61 aa until the next stop codon, each time the probability of read-through would decrease, but it is conceivable a fraction of the translated products have extra tails of amino-acids that should not be there. However, this will not result in more numerous Spike proteins, just extra-length on a fraction of them, whose biological impact is completely unknown. From a molecular biologist.
I don't have academic training but I have an inquiring mind. If our ribosomes evolved to make proteins on request from mRNA that has these specific stop codons, presumably to signal the ribosome that the docket is now fulfilled, is it reasonable to ask whether mRNA without these specific stop codons but with substitute [substandard] codons, can damage the ribosome?
Paul and all,
Says all you need to know today if you are an American!
https://newswithviews.com/the-dnc-is-the-ccp-of-the-usa/
P.S. Personally…I have worked with the NORTH AMERICAN LAW CENTER group for 15 years and can tell you they are America’s best brilliant and strategic minds!
Please help spread Lex’s pieces…important information ALL Americans need!
Reach out to Lex at his email found at the end of all his pieces to find out how to receive his information!
Come join with the best!
Regards,
A Mother/Grandmother Lion of 6 and counting
I wonder if there’s a risk of continued protein production in commercially sterilized fibrin sealants used in surgeries, or is it safe to assume that the “ground to a powder” production process would eliminate the spiked protein?
As production of the COVID-19 "vaccination" by Pfizer was actually an OTA contract between Pfizer and the Department of Defense, Pfizer was only required to produce a "demonstration" product. There was no party responsible for overseeing the entire operation (Trump's Operation Warpspeed) to insure safety and efficacy. The FDA and CDC roles were only a fake rubber stamp of approval. The "vaccine" functions well in its role as a medical countermeasure, aka bioweapon. Obviously, the health of we the people was never intended to be a factor of importance in the production and distribution of this poison. In addition to the world's population being a victim of this act of war, science has been discarded as a benefit to mankind.
My understanding is that the stop codon indicates the end of the protein. So stop and release what has been produced. The mRNA strand is then degraded and another produced from DNA if more protein is needed.
Adding the pseudouridine makes the breakdown of this mRNA strand difficult or impossible. Thus this strand is reused for another copy of protein.
If the cells cannot break it down, what happens to it? Presumably shedding could provide a mechanism for removal from the cells and body. Is there somewhere else in nature that this artificial mRNA will be degraded? I’d expect so, only outside of cell components where protein production occurs.