Makis deals with the mRNA technology gene based injection (Pfizer & Moderna), lipid-nano particle (LNP/mRNA) platform on how they impact bone marrow stem cells, affect their growth and differentiation
leading to possible turbo cancers such as leukemias, alters gene expression in bone marrow stem cells; LNP/mRNA technology is being combined with CRISPR tech for gene editing.
‘researchers are playing with “self-amplifying mRNA”, which means that the mRNA will now be able to replicate itself within YOUR cells so you get exponentially higher levels of spike protein produced to “improve vaccine efficacy”. As if we all need EVEN MORE spike protein.’
Makis’s scholarship on this topic is outstanding:
mRNA injury series - Pifzer & Moderna mRNA vaccines ATTACK bone marrow stem cells and drastically alter gene expression. Researchers are also using LNPs/mRNA for gene editing. Three studies reviewed.
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I review three recent papers:
Sep.7, 2023 - Zurlo et al - The anti-SARS-CoV-2 BNT162b2 vaccine suppresses mithramycin-induced erythroid differentiation and expression of embryo-fetal globin genes in human erythroleukemia K562 cells
Jul.27, 2023 - Breda et al - In vivo hematopoietic stem cell modification by mRNA delivery
Jan.22, 2023 - Puccetti et al - Biodrug Delivery Systems: Do mRNA Lipid Nanoparticles Come of Age?
Sep.7, 2023 - Zurlo et al - The anti-SARS-CoV-2 BNT162b2 vaccine suppresses mithramycin-induced erythroid differentiation and expression of embryo-fetal globin genes in human erythroleukemia K562 cells
Italian researchers treated K562 stem cells with increasing concentrations of Pfizer COVID-19 mRNA vaccine
What are K562 stem cells? - K-562 are lymphoblast cells isolated from the bone marrow of a 53-year-old chronic myelogenous leukemia patient. The K-562 cell line is widely used in immune system disorder and immunology research.
What are lymphoblast cells? Immature white blood cells that develop into healthy immune cells called lymphocytes. In leukemia, lymphoblasts don’t mature, instead they multiply rapidly in bone marrow and interfere with all blood cell production.
researchers were able to inhibit the growth of stem cells as Pfizer dose increased
Spike protein levels also increased with higher Pfizer mRNA doses
Spike protein increased expression of pro-inflammatory genes through up-regulation of NF-kB
Spike protein drastically decreased expression of several globin genes
Spike protein suppressed erythroid differentiation of stem cells
“The impact of SARS-CoV-2 Spike protein on cellular functions is of key interest”
“searching for circulating Spike in plasma of COVID-19 patient might help in understanding unexpected adverse effects following COVID-19 mRNA vaccination”
Conclusion: “SARS-CoV-2 S-protein, COVID-19 mRNA vaccines and SARS-CoV-2 infection might have dramatic effects of the hematopoietic compartment”
Conclusion: “need of great attention on possible alteration of hematopoietic parameters following SARS-CoV-2 infection and/or COVID-19 vaccination”
Spike protein production rises dramatically with increasing doses of Pfizer mRNA vaccine. This rise appears exponential.
Increasing doses of Pfizer mRNA vaccine cause dramatic suppression of globulin gene expression in bone marrow stem cells
Jul.27, 2023 - Breda et al - In vivo hematopoietic stem cell modification by mRNA delivery
This paper was published in “Science”
Summary: NIH funded authors injected LNPs/mRNA and delivered them to bone marrow stem cells where they conducted gene editing and “bone marrow transplantation”
The researchers developed two payloads: one that edited a mutation for sickle cell disease, and another that selectively killed hematopoietic stem cells, which would eliminate the need for chemotherapy before HSC transplantation.
If the therapies can be successfully adapted to people, this approach “will actually make gene therapy affordable, not only to our patients but also to our health care system,” says Hamideh Parhiz, a biotechnologist at the University of Pennsylvania, who co-led the research.
The researchers designed the lipid nanoparticles to target HSCs using an antibody that binds to the protein CD117, which is found on these cells’ surface.
After confirming that the nanoparticles were breaking through into about half of blood cells, they loaded the antibody-coated nanoparticles with an mRNA encoding a protein that induces cell death.
Although the nanoparticles killed HSCs, the researchers discovered some off-target effects, so they added tiny bits of noncoding RNA that kept the protein from killing other cells. “That’s when we got success,” Parhiz says.
In another experiment, the researchers stuffed their nanoparticles with an mRNA sequence that produces a gene editor when it enters the cell. The editor targets a mutation in hemoglobin causing sickle cell disease.
The researchers tested the gene-editing nanoparticles on cells grown from samples taken from people with the disease. Reversing the mutation resulted in more than 95% of blood cells taking on a typical round shape rather than the sickle-like appearance characteristic of the disease.
Parhiz and her colleagues are working on fine-tuning the approach and testing it further in animals to get a better understanding of how efficiently it edits intended genes and how well it targets HSCs.
The study is “an impressive advance,” says David R. Liu, a chemist and gene editing expert at the Broad Institute of MIT and Harvard. Though many steps remain before clinical testing, he says, the approach “could lay a foundation for the much broader availability of programmable therapeutic gene editing to treat a variety of genetic blood disorders”
“A step toward stem cell engineering in vivo”
Journal Intro: Hematopoietic stem cell (HSC) gene therapy provides lifelong and substantial benefits for several life-threatening inherited diseases, such as primary immunodeficiencies, storage disorders, and hemoglobinopathies.
Currently, HSC gene therapy requires harvesting large numbers of a patient's hematopoietic stem and progenitor cells (HSPCs), which undergo gene transfer or editing ex vivo. Before infusion, the cell product is qualified to ensure that it meets rigorous safety and efficacy standards, and the patient undergoes conditioning chemotherapy to deplete endogenous HSPCs and make space for the engineered cells to engraft in the bone marrow.
However, the need for laborious manufacturing and the toxicity associated with the conditioning regimen limits the broad application of these treatments.
On page 436 of this issue, Breda et al. provide a proof of principle of in vivo genetic engineering of HSPCs in the bone marrow of mice by leveraging transient delivery of mRNA through lipid nanoparticles (LNPs) functionally coupled to antibodies that target HSPCs.
Jan.22, 2023 - Puccetti et al - Biodrug Delivery Systems: Do mRNA Lipid Nanoparticles Come of Age?
“Although recent research has largely focused on advancing mRNA vaccines and large-scale manufacturing capabilities, the technology has also been used to develop various immunotherapies, gene editing strategies, and protein replacement therapies.”
“Lipid nanoparticles (LNPs) have emerged as a very promising delivery method. However, when intravenously delivering LNPs, most of the cargo is trapped by the liver”
Modifying the composition of the lipids in LNPs allows for the more specific delivery of the LNPs to some organs
“Messenger RNAs (mRNAs) present great potential as therapeutics for the treatment and prevention of a wide range of human pathologies, allowing for protein replacement, vaccination, cancer therapy, and genomic engineering”
mRNA for vaccines: “Optimal vaccine targets can be quickly discovered through genetic sequencing, rapidly yielding templates for subsequent large-scale mRNA production. The rapid discovery process, synergistically paired with relatively inexpensive biomanufacturing costs for LNP formulations, have enabled mRNA vaccine candidates to reach clinical testing and receive regulatory authorization much faster than traditional vaccines.”
Both Pfizer & Moderna mRNA vaccines “contain nucleoside-modified mRNAs that induce the membrane-bound expression of a perfusion-stabilized, full-length SARS-CoV-2 spike protein. In each case, the mRNA vaccines were formulated using LNPs for intramuscular injection. The rapid development and potent efficacy of these vaccines will serve as a strong benchmark for the advancement of future mRNA-based vaccines against a broad set of diseases.”
“to increase the efficacy of mRNA-based vaccines, additional strategies such as self-amplifying mRNA vaccines are being developed.
Self-amplifying mRNA vaccines use an engineered RNA virus genome in which the genes for the antigens of interest are inserted in place of those encoding the virus structural proteins while the genes for the virus RNA replication machinery are kept intact.
In contrast to traditional mRNA-based vaccines, self-amplifying mRNA vaccines allow for the intracellular replication of antigen-encoding RNA, resulting in a higher level of antigen production that enhances vaccine efficacy.
Self-amplifying mRNA vaccines show some difficulties compared with mRNA vaccines.
They have a necessarily higher molecular size due to the presence of the viral-derived genes for the RNA replication machinery, which can also cause immunogenicity, thus limiting their potential repeated use
Thus far, the self-amplifying mRNA vaccine platform has been applied against diverse viruses including influenza, Ebola, hepatitis C, rabies virus, Toxoplasma gondii, human cytomegalovirus, and HIV-1.
mRNA for Gene Editing: “In addition to protein replacement and vaccines, more recently, the development of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) technology led to the application of mRNAs in gene editing and extended their use in pathologies requiring not only protein expression but also gene knockout”
My Take…
We know from the Japan biodistribution study obtained by Virologist Dr.Byram Bridle, that Pfizer COVID-19 mRNA vaccine accumulates in the bone marrow
On May 27, 2021, Moderna’s Fourth Annual Science Day, Moderna boasted about their ability to deliver mRNA to the bone marrow, causing “long term modulation of all hematopoietic lineages” on page 113 of a document that is now impossible to find (click here):
KEY POINTS from Zurlo et al paper:
Pfizer COVID-19 mRNA vaccine accumulates in the bone marrow and can inhibit the growth & suppress the differentiation of bone marrow stem cells
Pfizer spike protein can drastically alter gene expression in stem cells
Pfizer spike protein can increase expression of pro-inflammatory genes
spike protein production in bone marrow stem cells increases dramatically with increasing mRNA dose (looks exponential)
authors conclude: “Pfizer spike protein might have dramatic effects on the hematopoietic compartment”
KEY POINTS from Breda et al and Puccetti et al papers:
LNPs/mRNA can be delivered to bone marrow stem cells where they conduct gene editing and bone marrow transplantation
LNPs can be modified via a surface “decoration” to improve targeted delivery of mRNA cargo
mRNA can be encoded with a protein that induces bone marrow cell death
mRNA can also be encoded with sequence that produces a “gene editor” when it enters the cell
LNP/mRNA is repeatedly referred to as “gene therapy” and a platform for “genetic engineering” including “gene editing strategies”.
My Concerns…
All these recent papers downplay the dangers of the LNP/mRNA platform and completely ignore the millions of COVID-19 mRNA vaccine injuries & deaths, pretending they are not happening as they push forward
COVID-19 mRNA vaccines are being referred to as a “resounding success” even though they are a complete failure.
Pfizer COVID-19 mRNA vaccine messes with bone marrow stem cells, affects their growth and differentiation, the clinical implications of which we don’t understand. Can this lead to turbo cancers such as leukemias?
Pfizer COVID-19 mRNA vaccine alters gene expression in bone marrow stem cells the clinical implications of which we don’t understand.
Spike protein production in stem cells is not linear - slightly more mRNA can lead to exponentially higher spike protein production - may partly explain severity of COVID-19 mRNA vaccine injuries in someone who may have received only slightly higher concentration of mRNA in their vaccine dose.
LNP/mRNA is a gene therapy, and a platform for “genetic engineering” including “gene editing strategies”.
Slight modification of LNP’s external “decoration” can drastically impact where LNPs get delivered. Researchers are already playing with these modifications.
LNP/mRNA technology is being combined with CRISPR tech for gene editing.
researchers are playing with “self-amplifying mRNA”, which means that the mRNA will now be able to replicate itself within YOUR cells so you get exponentially higher levels of spike protein produced to “improve vaccine efficacy”. As if we all need EVEN MORE spike protein.’
Insane, dialing up the Cyborg future... for those blind enough to go along with being turned into a Biological Time Bomb.. We must Wake Up as many as we can... education in the truest sense.. We Can ... Nuremberg will be Realized and this Insane type of research will be terminated.. Fuuuchi and the entire Psyop team will go on trial... let’s do our part today... the Future is NOW
Wasn't Makis and FLCCC smeared recently by oncologist and "fact checker" David Gorski? If the 20 Horsemen are ever expanded to 24 ("the dirty two dozen") then Gorski should get a saddle. See the claim below attributed to Gorski, and then the article at the following link regarding syringe aspiration, or non-aspiration, implications.:
“If it’s [mRNA COVID pseudovaccine] injected into the muscle, most of the vaccine (sic) stays in a relatively small area. It doesn’t really go very far,” Gorski told USA TODAY. “If you inject it into the bloodstream, it’s in the bloodstream – it’s going everywhere. … Nothing like what is done in real life.”
Claim linking Pfizer COVID-19 vaccine, cancer in mouse distorts study | Fact check
https://www.usatoday.com/story/news/factcheck/2023/08/30/post-falsely-links-pfizer-covid-19-vaccine-mouse-cancer-fact-check/70702575007/
Gorski deceitfully claims that "most of the vaccine (sic) stays in a relatively small area. It doesn’t really go very far.”
There is accumulating evidence that claim is not true, or at least not in all cases.
And Gorski, unlike Democrat commenter on these stacks, "scout," possibly a bot, and unlike pro-vax commenter "Wayne E" is, as a professor of medicine, likely of at least average intelligence.
He would know the evidence that his claim is false exists.
Gorski also claims, correctly, that: “If you inject it into the bloodstream, it’s in the bloodstream – it’s going everywhere."
But then Gorski adds, falsely, that this is "Nothing like what is done in real life.”
Gorski would know this is false, but like the shills for the tobacco companies of old, he deceitfully makes the statement, to mislead the recipient of the disinformation.
Gorski, like pro-jabbers generally, deals in cherry-picking, selective citation of information, andhiding, or omission of, inconvenient evidence.
Along with a broad selection of fallacious reasoning techniques, these are among the routinely used tools of Gorski's trade.
Gorski omits to note that there has been at least a small proportion of injections which, out of many millions of injections globally and in the US, is a very large number of them, in which the "vaccines" were injected into the bloodstream.
See the excerpts from the paper at the link below.
It matters not that the authors were concerned principally about the implications of injection into the bloodstream for myocarditis.
Implications for other possible ill health outcomes were not considered.
It does not mean that there aren't any.
(Gorski appears to assume that absence of evidence, or absence of conclusive proof, is the same thing as evidence of absence.)
"This [syringe aspiration] technique was specifically developed in the past to ensure the medication is not inadvertently delivered into a blood vessel. Before the pandemic, the aspiration has generated numerous discussions and controversies, with no conclusive evidence to understand whether such a procedure is beneficial or unwarranted due to the absence of randomized clinical trials."
"Syringe aspiration when vaccinating intramuscularly was not recommended before the pandemic due to the lack of conclusive evidence that it provides any benefit. However, in vivo evidence suggests that intravenous injection of mRNA vaccine can potentially lead to [ill health outcomes] ... syringe aspiration ... represents a simple technique to decrease the risk of vaccine introduction into the vascular system and potentially decrease the risk of severe reactions to mRNA and adenoviral vaccines. We are of the opinion that this cannot be disregarded if one considers that the COVID-19 vaccines will continue to be administrated globally in the form of initial and booster doses. Therefore, the aspiration when giving mRNA and adenoviral vaccines appears to be fully in line with the precautionary principle."
"Aspiration is a technique practiced to avoid accidental vaccine injection into a vessel during intramuscular administration. The appearance of blood in the syringe indicates that this is a case and shall result in another vaccination attempt. In this situation, the needle should be withdrawn, the syringe discarded, and another injection (prepared using new vaccine dose and equipment) should be given in a different location [17, 23]. The deltoid muscle is the preferred injection site for SARS-CoV-2 vaccines. Although the usual spot, 5–7 cm below the acromion, is relatively distant from big vessels, in some cases, the posterior circumflex humeral artery can be present in this area [24]. But even if major blood vessels in their typical locations are not in immediate proximity, anatomical variants and smaller branches can cause accidental intravessel administration of the vaccine or a part of it. While it may not represent a significant risk for various vaccines approved pre-pandemic and based on a more classical approach, it is not necessarily a case about mRNA and adenoviral vector vaccines, which administration was limited to clinical trials before the COVID-19 pandemic. In 2021, over 2.5 billion doses of mRNA vaccines and 2.5 billion doses of adenoviral vector vaccines were globally given to humans [3]. In turn, evidence from experiments in vivo highlights that introducing both mRNA and adenoviral vector vaccines into the blood instead of muscle can result in acute adverse events resembling those seen in post-authorization pharmacovigilance for humans given the same vaccines."
To aspirate or not to aspirate? Considerations for the COVID-19 vaccines
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8941363/
Professor Norman Fenton has noted:
"The standard procedure of ‘aspiration’ for intramuscular injections was generally stopped for the covid vaccines (although some countries like Denmark reverted to the practice after serious adverse reactions were caused by lack of aspiration) [54] and therefore it is likely that approximately 2% of vaccinations went straight into the blood, potentially explaining why a small proportion of vaccine recipients suffered far worse than most [55]."
Informed consent: statement on covid policies affecting children and young adults
https://wherearethenumbers.substack.com/p/informed-consent-statement-on-covid?utm_campaign=email-post&r=1gmx8e&utm_source=substack&utm_medium=email
Approximately one in 50.jabtards have been injected with a novel mRNA anti-COVID into their bloodstream. That's an enormous number of people, both globally and in the US.
And yet, despite the implications, there is no reason to believe that Gorski has ever taken action to mitigate potential harms.
Instead, Gorski, a purported "fact checker," peddles disinformation
Is this not recklessly endangering the public?
Gorski's tactics are analogous to tobacco companies placing the onus on smokers to prove that cigarette smoking is harmful, rather than placing the onus on tobacco companies to prove that it is not.
Nobody, however, has ever been mandated to smoke cigarettes.
This arguably makes Gorski's behavior even more morally reprehensible than it would otherwise be.
Don't allow yourself to be influenced by grifters.
Here is an article about when cigarette companies used doctors to push smoking, in an analogous way to how some believe pharmaceutical companies use the likes of Gorski to push the new mRNA pseudovaccines.
When Cigarette Companies Used Doctors to Push Smoking. Before studies showed that cigarettes caused cancer, tobacco companies recruited the medical community for their ads.
https://www.history.com/news/cigarette-ads-doctors-smoking-endorsement
It is disgraceful that good doctors like Makis are being persecuted while others in the pro-jabs camp like Gorski are demonstrably peddling disinformation.